LC-MS/MS Quantification of Proteins CD47 and SIRPA in Cell Subtypes Selected from Blood by Flow Cytometry, presented at the ACCR Annual Meeting, April 5-9, 2014 in San Diego, CA.

Flow cytometry and mass spectrometry are widely used analytical tools in cancer research. Flow cytometry is well established for cell counting, cell sorting and cancer biomarker discovery. Mass spectrometry is unparalleled in its ability to selectively detect and quantify target analytes at the molecular level. The combination of flow cytometry to isolate specific cell subtypes from biological fluids such as blood, and mass spectrometry to quantify proteins contained in the selected cells, provides a new approach to studying protein expression in specific cell subtypes. We have used this combination to investigate the normal levels of CD47 and SIRPA proteins in CD8+ T-cells, CD4+ Tcells, CD14+ monocytes, CD3+ myeloid cells and CD56+ NK cells isolated from blood specimens as a first step toward better understanding of how the CD47/SIRPA protein levels in these cells are affected by cancer and cancer treatment.


To gain access to full literature please fill out this form.

[contact-form-7 404 "Not Found"]